| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bone sialoprotein 2; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein; IBSP; BNSP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human IBSP, which shares 88.9% and 87% amino acid (aa) sequence identity with mouse and rat IBSP, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets integrin-binding sialoprotein (gene IBSP) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 35.1 kDa, and the supplier reports an observed band at about 65 kDa.
Integrin-binding sialoprotein (IBSP, also called bone sialoprotein) is a highly glycosylated protein of the SIBLING family found in mineralized tissues such as bone, dentin and calcified cartilage. It binds tightly to hydroxyapatite and supports cell attachment through the αvβ3 integrin, and it is a common marker of osteoblast differentiation and bone formation.
| Target | Integrin-binding sialoprotein (gene IBSP; UniProt P21815, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 35.1 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human placenta tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human IBSP. Sequence identity with the mouse and rat orthologs: 88.9% and 87%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes integrin-binding sialoprotein as a secreted protein. Tissue expression noted by UniProt (human): Expressed in bone (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Keqing Huang et al. (2019) Halloysite Nanotube Based Scaffold for Enhanced Bone Regeneration. ACS Biomaterials Science & Engineering. 10.1021/acsbiomaterials.9b00277
Xiaobo Wei et al. (2024) Enhancing osteoblast proliferation and bone regeneration by poly (amino acid)/selenium-doped hydroxyapatite. Biomedical Materials. 10.1088/1748-605X/ad38ac
Du Jing et al. (2020) Effects of ERK/p38 MAPKs signaling pathways on MTA-mediated osteo/odontogenic differentiation of stem cells from apical papilla: a vitro study. BMC Oral Health. 10.1186/s12903-020-1016-x
