| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human ICAM1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody is an antibody targeting ICAM1. Common applications include WB, IHC, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DOD-9; isotype: Rabbit IgG; reactivity: Human; observed MW: 100 kDa; calculated MW: 57825 MW.
Boster Bio Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody catalog # M00171-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: ICAM1 — Intercellular adhesion molecule 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DOD-9; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 100 kDa; Calculated: 57825 MW
Biological background
Protein function (datasheet): ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .
Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. .
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Atherosclerosis,Cardiovascular,Cell Adhesion Molecules,Cell Type Markers,Hematopoietic Progenitors,Immunology,Lymphoid,Stem Cells,T Lymphocytic Lineage,Vascular Inflammation.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
