| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-binding protein inhibitor ID-2;Class B basic helix-loop-helix protein 26;bHLHb26;Inhibitor of DNA binding 2;Inhibitor of differentiation 2;ID2;BHLHB26; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ID2 recombinant protein (Position: M1-C133). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA-binding protein inhibitor ID-2 (gene ID2) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 14.9 kDa, and the supplier reports an observed band at about 18 kDa.
ID2 (inhibitor of DNA binding 2) is a helix-loop-helix protein without a DNA-binding domain that forms inactive dimers with basic helix-loop-helix transcription factors. By blocking their activity it regulates cell growth and differentiation in many tissues, including neural development and the specification of innate lymphoid and NK cells.
| Target | DNA-binding protein inhibitor ID-2 (gene ID2; UniProt Q02363, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 14.9 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HepG2 cells, human Caco-2 cells, human U87 cells, human PC-3 cells, human HL-60 cells, human HeLa cells, rat liver tissue, rat ovary tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human ID2 fragment (Met1–Cys133), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-binding protein inhibitor ID-2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in early fetal tissues, including those of the central nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
