Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody

Overview

Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody is an antibody targeting IGHG4. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DCF-9; isotype: Rabbit IgG; reactivity: Human; observed MW: 14-36 kDa; calculated MW: 35941 MW.

Boster Bio Anti-IgG4 IGHG4 Rabbit Monoclonal Antibody catalog # M11385-1. Tested in WB, IHC applications. This antibody reacts with Human.

Key elements and design rationale

• Target: IGHG4 — Ig gamma-4 chain C region
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DCF-9; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 14-36 kDa; Calculated: 35941 MW

Biological background

Protein function (datasheet): Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Cellular localization (datasheet): Secreted.

Tissue details (datasheet): Highly expressed in skeletal muscle, heart, liver and testis. .

Research relevance and current trends

• Commonly studied in contexts related to Immunoglobulins,Immunology.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.