Anti-IKK beta/IKBKB Antibody Picoband®

Overview

Anti-IKK beta/IKBKB Antibody Picoband® is an antibody targeting IKBKB. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 87 kDa; calculated MW: 86564 MW.

Boster Bio Anti-IKK beta/IKBKB Antibody catalog # PA2036-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Target: IKBKB — Inhibitor of nuclear factor kappa-B kinase subunit beta
• Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 87 kDa; Calculated: 86564 MW

Specificity note: No cross reactivity with other proteins.

Biological background

Protein function (datasheet): Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. .

Scientific background (datasheet): IKBKB (Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB , is a protein that in humans is encoded by the IKBKB gene. Shindo et al. (1998) mapped the IKBKB gene to chromosome 8p12-p11 by FISH. Mercurio et al. (1997) found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. Yin et al. (1998) tested the activity of various antiinflammatory agents on the IKK complex. They demonstrated that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.

Cellular localization (datasheet): Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.

Tissue details (datasheet): Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

Sequence similarities (datasheet): Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Research relevance and current trends

• Commonly studied in contexts related to Atherosclerosis,Cardiovascular,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,NFKB Pathway,Nuclear Signaling Pathways,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,TLR Signaling,Vascular Inflammation.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Cross-reactivity (datasheet): No cross-reactivity with other proteins
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.