| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NF-kappa-B essential modulator;NEMO;FIP-3;IkB kinase-associated protein 1;IKKAP1;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;IKBKG;FIP3, NEMO; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human IKK gamma |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-IKBKG antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABCA-9; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-IKK gamma IKBKG Rabbit Monoclonal Antibody catalog # M00874. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: IKBKG (NF-kappa-B essential modulator).
- Antibody format: Monoclonal; clone ABCA-9; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
IKBKG (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. . Reported cellular localization context: Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress. Tissue expression notes (as provided): Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Research relevance and current trends
- Research context keywords from the source record include: Apoptosis,Apoptotic Markers,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Death,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Intracellular,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway,TLR Signaling,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate IKBKG antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect IKBKG expression by Western blot in cell or tissue lysates, Detect IKBKG in FFPE tissue sections by immunohistochemistry, Localize IKBKG by immunofluorescence/immunocytochemistry in cultured cells, Quantify IKBKG-positive cells by flow cytometry in single-cell suspensions, Enrich IKBKG by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 200 kDa; calculated MW: 48198 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 200 kDa
- Cellular localization (provided): Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
- Tissue details (provided): Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
