| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-2;IL-2;T-cell growth factor;TCGF;Aldesleukin;IL2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human IL-2 recombinant protein (Position: A21-T153). Human IL-2 shares 57.5% and 66.4% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IL2 (Interleukin-2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IL-2/IL2 Antibody Picoband® catalog # A00387-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human IL-2 recombinant protein (Position: A21-T153). Human IL-2 shares 57.5% and 66.4% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. (reported region: A21-T153).
- Molecular weight context: reported MW: 15 kDa; calculated MW: 18 kDa
- Reactivity: Human
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Interleukin-2; Interleukin-2. IL-2, Interleukin-2, formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. In situ hybridization, the IL-2 gene is assigned to 4q26-q28. IL2 can act as a growth hormone for both B and T lymphocytes. It is useful in the study of the molecular nature of T-cell differentiation and, like interferons, augments natural killer cell activity. Functional note: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. Reported localization: Secreted. Expression/tissue context: Expressed in fetal brain.
Research relevance and current trends
- Adaptive Immunity: Researchers commonly examine how IL2 (Interleukin-2) relates to this theme using model systems and orthogonal readouts.
- Cell Type Markers: Researchers commonly examine how IL2 (Interleukin-2) relates to this theme using model systems and orthogonal readouts.
- Cytokines: Researchers commonly examine how IL2 (Interleukin-2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative IL2 (Interleukin-2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of IL2 (Interleukin-2) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
