| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-2;IL-2;T-cell growth factor;TCGF;Aldesleukin;IL2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL-2 recombinant protein (Position: A21-T153). Human IL-2 shares 57.5% and 66.4% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interleukin-2 (gene IL2) in human samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 17.6 kDa, and the supplier reports an observed band at about 15 kDa.
Interleukin-2 (IL-2) is a cytokine made mainly by activated CD4+ T cells. Acting through the IL-2 receptor and JAK1/JAK3–STAT5 signaling, it drives T-cell proliferation, boosts NK-cell killing and supports B-cell responses, while also sustaining regulatory T cells that keep immune tolerance in check. It is a standard marker of T-cell activation in immunology research.
| Target | Interleukin-2 (gene IL2; UniProt P60568, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 17.6 kDa |
| Observed MW | 15 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): recombinant human IL-2 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human IL-2 fragment (Ala21–Thr153), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 57.5% and 66.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interleukin-2 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Yan et al. (2020) Key Role of Pro-inflammatory Cytokines in the Toxic Effect of Fluoride on Hepa1-6 Cells. Biological Trace Element Research. 10.1007/s12011-019-01967-1
Deyuan Tang et al. (2014) Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine. Journal of Veterinary Science. 10.4142/jvs.2014.15.1.99
Liu Shu-lei et al. (2013) Effects of recombinant hIL-2-Luffin P1 immunotoxin combining with arotinoid ethylester on the proliferation and apoptosis of Hut-78 cells. Molecular & Cellular Toxicology. 10.1007/s13273-012-0041-5
