| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-22;IL-22;Cytokine Zcyto18;IL-10-related T-cell-derived-inducible factor;IL-TIF;IL22;ILTIF, ZCYTO18;UNQ3099/PRO10096; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interleukin-22 (gene IL22) in human, mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 20.0 kDa, and the supplier reports an observed band at about 24 kDa.
Interleukin-22 (IL-22) is an IL-10 family cytokine produced by immune cells such as Th17, Th22 and innate lymphoid cells, yet it acts mainly on epithelial and other non-immune cells. Through IL-22R1 and IL-10R2 it activates JAK1/TYK2 and STAT3, promoting epithelial survival, regeneration and barrier defense in gut, skin and lung.
| Target | Interleukin-22 (gene IL22; UniProt Q9GZX6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.0 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| ELISA (capture antibody) | 1–5 µg/mL (Human) |
Samples with a confirmed band (WB): human placenta tissue, rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectum adenocarcinoma tissue, human spleen tissue, mouse thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human IL-22 fragment (Ala34–Ile179), expressed in E. coli. Sequence identity with the mouse ortholog: 81.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes interleukin-22 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
