| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; Csf2rb; Aic2b; Csf2rb1; Il3rb1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouseIL-3RB/Csf2rb recombinant protein (Position: A28-Q891). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IL-3RB/Csf2rb Antibody Picoband® is an antibody for Csf2rb detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Csf2rb (colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)); UniProt: P26955
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 120 kDa, calculated 38160 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-IL-3RB/Csf2rb Antibody Picoband® catalog # A02219-3.
Biological background
Biological context: High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Expression and localization notes: cellular localization: Membrane. Single-pass type I membrane protein., tissue context: Broadly expressed with higher expression in heart..
Common research applications
- Western blotting (WB): Compare Csf2rb levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Csf2rb in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Csf2rb-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane. Single-pass type I membrane protein.
- Tissue details: Broadly expressed with higher expression in heart.
- Research category: Auditory System,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Neurogenesis,Neurology Process,Neuroscience,Nuclear,Sensory System,Signaling Pathways,Stem Cells,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
