| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-1 beta; IL-1 beta; Il1b; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse IL-1 beta/Il1b recombinant protein (Position: V118-S269). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-1 beta (gene Il1b) in mouse samples and is validated for ELISA and Western blot. The predicted molecular weight is 30.9 kDa, and the supplier reports an observed band at about 36 kDa.
Interleukin-1 beta (IL-1β) is a potent pro-inflammatory cytokine made as an inactive precursor and processed by caspase-1 after inflammasome activation. Acting through IL1R1 and IL1RAP, it induces fever, prostaglandin synthesis, neutrophil recruitment and Th17 differentiation, and it is one of the most studied mediators of inflammation.
| Target | Interleukin-1 beta (gene Il1b; UniProt P10749, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.9 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse RAW2647(-LPS) cells, mouse RAW2647(+LPS) cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse IL-1 beta/Il1b fragment (Val118–Ser269), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes interleukin-1 beta as secreted and also located in the cytoplasm and lysosome. Tissue expression noted by UniProt (mouse): Expressed in activated macrophages (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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