| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-13;IL-13;IL13;NC30; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human IL13 recombinant protein (Position: P36-N146). Human IL13 shares 55.3% and 61.9% amino acid (aa) sequence identity with mouse and rat IL13, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IL13 (Interleukin-13) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IL13 Antibody Picoband® catalog # A00077. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human IL13 recombinant protein (Position: P36-N146). Human IL13 shares 55.3% and 61.9% amino acid (aa) sequence identity with mouse and rat IL13, respectively. (reported region: P36-N146).
- Molecular weight context: reported MW: 20 kDa; calculated MW: 15816 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Interleukin-13; Interleukin-13. Interleukin 13 is a protein that in humans is encoded by the IL-13 gene. It is a kind of cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, which is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. Furthermore, this gene acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function. Functional note: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Reported localization: Secreted. Expression/tissue context: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
Research relevance and current trends
- Cytokines: Researchers commonly examine how IL13 (Interleukin-13) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how IL13 (Interleukin-13) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how IL13 (Interleukin-13) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative IL13 (Interleukin-13) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of IL13 (Interleukin-13) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
