| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-13;IL-13;IL13;NC30; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL13 recombinant protein (Position: P36-N146). Human IL13 shares 55.3% and 61.9% amino acid (aa) sequence identity with mouse and rat IL13, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interleukin-13 (gene IL13) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 15.8 kDa, and the supplier reports an observed band at about 20 kDa.
Interleukin-13 (IL-13) is a type 2 cytokine produced by Th2 cells, group 2 innate lymphoid cells and mast cells. It signals mainly through the IL-4Rα/IL13RA1 receptor and STAT6, driving mucus production, airway hyperresponsiveness and tissue remodeling, and it is a central mediator in allergic inflammation, asthma and anti-parasite immunity.
| Target | Interleukin-13 (gene IL13; UniProt P35225, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 15.8 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human IL13 fragment (Pro36–Asn146), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 55.3% and 61.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interleukin-13 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bihui Wang et al. (2024) Hyperactivity of the IL-33-ILC2s-IL-13-M-MDSCs axis promotes cervical cancer progression. International Immunopharmacology. 10.1016/j.intimp.2024.113693
Jian Li et al. (2022) Interleukin 13 participates in terminal differentiation of esophageal squamous cell carcinoma cells. Journal of Gastrointestinal Oncology. 10.21037/jgo-22-559
