| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-1 receptor antagonist protein;IL-1RN;IL-1ra;IRAP;ICIL-1RA;IL1 inhibitor;Anakinra;IL1RN;IL1F3, IL1RA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human IL1RA recombinant protein (Position: R26-E177). Human IL1RA shares 77% and 75.5% amino acid (aa) sequence identity with mouse and rat IL1RA, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IL1RN (Telomeric repeat-binding factor 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IL1RA/IL1RN Antibody Picoband® catalog # A00651-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human IL1RA recombinant protein (Position: R26-E177). Human IL1RA shares 77% and 75.5% amino acid (aa) sequence identity with mouse and rat IL1RA, respectively. (reported region: R26-E177).
- Molecular weight context: reported MW: 20 kDa; calculated MW: 20055 MW
- Reactivity: Human
- Applications: ELISA, IHC, WB, Flow Cytometry
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Telomeric repeat-binding factor 2; Interleukin-1 receptor antagonist protein. The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. Functional note: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. . Reported localization: Isoform 1: Secreted. Expression/tissue context: The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Research relevance and current trends
- Cytokines: Researchers commonly examine how IL1RN (Telomeric repeat-binding factor 2) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how IL1RN (Telomeric repeat-binding factor 2) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how IL1RN (Telomeric repeat-binding factor 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative IL1RN (Telomeric repeat-binding factor 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of IL1RN (Telomeric repeat-binding factor 2) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
