Anti-IL2 Receptor beta/IL2RB Antibody Picoband®

Overview

This antibody is intended for detection of Il2rb (Interleukin-2 receptor subunit beta) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-IL2 Receptor beta/IL2RB Antibody Picoband® catalog # PB9761. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL2RB, different from the related human sequence by sixteen amino acids, and from the related rat sequence by fourteen amino acids.
• Molecular weight context: reported MW: 75 kDa; calculated MW: 60539 MW
• Reactivity: Mouse
• Applications: WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Interleukin-2 receptor subunit beta; Interleukin-2 receptor subunit beta. IL2RB (INTERLEUKIN 2 RECEPTOR, BETA), also called CD122, is a protein that in humans is encoded by the IL2RB gene. The gene represents the beta subunit and is a type I membrane protein. IL2RB is mapped to 22q12.3. The IL2RB gene contains 10 exons spanning about 24.3 kb and that the gene product is encoded by exons 2-10. The IL2 receptor is present in 3 forms with respect to binding ability to IL2: high, intermediate, and low affinity forms. Analysis of mutants of the beta chain of the IL2 receptor reveals that the granulocyte and monocyte differentiation signals are triggered by different cytoplasmic domains, showing that the signaling pathways responsible for these unique developmental outcomes are separable. Functional note: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Reported localization: Membrane; Single-pass type I membrane protein. Expression/tissue context: Expressed in lung (at protein level). Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain. .

Research relevance and current trends

• Adaptive Immunity: Researchers commonly examine how Il2rb (Interleukin-2 receptor subunit beta) relates to this theme using model systems and orthogonal readouts.
• Cytokines: Researchers commonly examine how Il2rb (Interleukin-2 receptor subunit beta) relates to this theme using model systems and orthogonal readouts.
• Hematopoietic Progenitors: Researchers commonly examine how Il2rb (Interleukin-2 receptor subunit beta) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative Il2rb (Interleukin-2 receptor subunit beta) levels across conditions; band patterns may reflect isoforms and processing.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.