| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-2 receptor subunit beta;IL-2 receptor subunit beta;IL-2R subunit beta;IL-2RB;High affinity IL-2 receptor subunit beta;p70-75;CD122;Il2rb; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL2RB, different from the related human sequence by sixteen amino acids, and from the related rat sequence by fourteen amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interleukin-2 receptor subunit beta (gene Il2rb) in mouse samples and is validated for Western blot. The predicted molecular weight is 60.5 kDa, and the supplier reports an observed band at about 75 kDa.
IL-2 receptor beta (CD122) is a signaling subunit shared by the receptors for IL-2 and IL-15. Together with the common gamma chain, it recruits JAK1 and activates STAT5 to relay growth and survival signals to T cells and NK cells, and it also mediates receptor internalization.
| Target | Interleukin-2 receptor subunit beta (gene Il2rb; UniProt P16297, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.5 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|
Samples with a confirmed band (WB): mouse lung tissue, mouse spleen tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse IL2RB. It differs from the human sequence at sixteen residues and from the rat sequence at fourteen residues.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places interleukin-2 receptor subunit beta in the cell membrane and cell surface. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
