Anti-IL2 Receptor beta/IL2RB Antibody Picoband®

Overview

Anti-IL2 Receptor beta/IL2RB Antibody Picoband® is an antibody targeting IL2RB. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 75 kDa; calculated MW: 61117 MW.

Boster Bio Anti-IL2 Receptor beta/IL2RB Antibody catalog # PA1615. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Target: IL2RB — Interleukin-2 receptor subunit beta
• Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 75 kDa; Calculated: 61117 MW

Specificity note: No cross reactivity with other proteins.

Biological background

Protein function (datasheet): Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Scientific background (datasheet): IL2RB (INTERLEUKIN 2 RECEPTOR, BETA),also called CD122, is a protein that in humans is encoded by the IL2RB gene. The gene represents the beta subunit and is a type I membrane protein. IL2RB is mapped to 22q12.3. The IL2RB gene contains 10 exons spanning about 24.3 kb and that the gene product is encoded by exons 2-10. The IL2 receptor is present in 3 forms with respect to binding ability to IL2: high, intermediate, and low affinity forms. Analysis of mutants of the beta chain of the IL2 receptor reveals that the granulocyte and monocyte differentiation signals are triggered by different cytoplasmic domains, showing that the signaling pathways responsible for these unique developmental outcomes are separable.

Cellular localization (datasheet): Membrane; Single-pass type I membrane protein.

Sequence similarities (datasheet): Belongs to the type I cytokine receptor family. Type 4 subfamily.

Research relevance and current trends

• Commonly studied in contexts related to Adaptive Immunity,Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Stem Cells,Tyrosine Kinases.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Cross-reactivity (datasheet): No cross-reactivity with other proteins
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.