| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL22; ILTIF, ZCYTO18; UNQ3099/PRO10096 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of IL22 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IL22 Antibody Picoband® (monoclonal, 7F2) catalog # M00963. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Mouse Monoclonal Mouse IgG1
- Clone number: Clone: 7F2
- Immunogen / epitope context: E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. (reported region: A34-I179).
- Molecular weight context: reported MW: 24 kDa; calculated MW: nan
- Reactivity: Human
- Applications: Flow Cytometry, WB
As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.
Biological background
interleukin 22. Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A. Functional note: Cytokine that contributes to the inflammatory response in vivo. Reported localization: Secreted.
Research relevance and current trends
- Adapters: Researchers commonly examine how IL22 relates to this theme using model systems and orthogonal readouts.
- Cytokines: Researchers commonly examine how IL22 relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how IL22 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative IL22 levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
