| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; Interleukin-30; p28; IL27; IL27A; IL30 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL27 recombinant protein (Position: F29-P243). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-27 subunit alpha (gene IL27) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 27.5 kDa, and the supplier reports an observed band at about 60 kDa.
IL-27 p28 (IL27, also IL-30) is the subunit that pairs with EBI3 to form the heterodimeric cytokine IL-27. Acting through the WSX-1/gp130 receptor and STAT1/STAT3 signaling, IL-27 has both pro- and anti-inflammatory effects: it favors early Th1 responses, restrains Th2 and Th17 differentiation, and helps limit excessive T-cell activation.
| Target | Interleukin-27 subunit alpha (gene IL27; UniProt Q8NEV9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 27.5 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): recombinant human IL27 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human IL27 fragment (Phe29–Pro243), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes interleukin-27 subunit alpha as a secreted protein. Tissue expression noted by UniProt (human): Expressed in monocytes and in placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
