| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; CD antigen CD25; Il2ra; Il2r |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Il2ra recombinant protein (Position: E22-I268). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interleukin-2 receptor subunit alpha (gene Il2ra) in mouse and rat samples and is validated for IHC, ICC/IF and ELISA. The predicted molecular weight is 30.7 kDa.
IL-2 receptor alpha (CD25) is the subunit that gives the IL-2 receptor high affinity for IL-2. It is highly expressed on regulatory T cells and on activated T cells, and it is essential for Treg maintenance and immune tolerance. CD25 is a common marker for Tregs and T-cell activation, and its soluble form is measured in inflammation studies.
| Target | Interleukin-2 receptor subunit alpha (gene Il2ra; UniProt P01590, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.7 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): mouse thymus tissue, rat thymus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): RAW264.7 cells.
Immunogen
Recombinant mouse Il2ra fragment (Glu22–Ile268), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places interleukin-2 receptor subunit alpha in the membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wong Victoria A. et al. (2024) IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells. Frontiers in Immunology. 10.3389/fimmu.2024.1369818
