| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-33; IL-33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV; Interleukin-33 (95-270); Interleukin-33 (99-270); Interleukin-33 (109-270); IL33; C9orf26, IL1F11, NFHEV; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human IL33 recombinant protein (Position: A95-T270). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects interleukin-33 (gene IL33) in human samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 18 kDa.
Interleukin-33 (IL-33) is an IL-1 family cytokine that acts as an “alarmin” released from damaged epithelial and endothelial cells. Binding to its receptor ST2 (IL1RL1) activates NF-κB and MAPK signaling, promoting type 2 immunity through Th2 cells, mast cells, eosinophils and group 2 innate lymphoid cells. Inside the nucleus, full-length IL-33 also associates with chromatin.
| Target | Interleukin-33 (gene IL33; UniProt O95760, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 30.8 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human tonsil.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human IL33 fragment (Ala95–Thr270), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interleukin-33 in the nucleus, chromosome, cytoplasm and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Vincze Anett et al. (2023) Pruritogenic molecules in the skin of patients with dermatomyositis. Frontiers in Medicine. 10.3389/fmed.2023.1168359
