| Field | Specification |
|---|---|
| Target | |
| Alternative names | Indoleamine 2,3-dioxygenase 1; IDO-1; Indoleamine-pyrrole 2,3-dioxygenase; IDO1; IDO; INDO |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human INDO |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17I51 is a rabbit monoclonal antibody that recognizes indoleamine 2,3-dioxygenase 1 (gene IDO1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 45.3 kDa, and the supplier reports an observed band at about 45 kDa.
IDO1 (indoleamine 2,3-dioxygenase 1) catalyzes the first and rate-limiting step in breaking down tryptophan along the kynurenine pathway. By depleting tryptophan and producing kynurenine metabolites in tissues and tumors, it suppresses T-cell responses and promotes regulatory T cells, so it is widely studied in immune tolerance, infection and tumor immune evasion.
| Target | Indoleamine 2,3-dioxygenase 1 (gene IDO1; UniProt P14902, human) |
|---|---|
| Clone | 17I51 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.3 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human INDO.
Reactivity Notes
The supplier lists reactivity with human. UniProt places indoleamine 2,3-dioxygenase 1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
