| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Indoleamine 2,3-dioxygenase 2;IDO-2;1.13.11.-;Indoleamine 2,3-dioxygenase-like protein 1;Indoleamine-pyrrole 2,3-dioxygenase-like protein 1;IDO2;INDOL1; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human INDOL1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-INDOL1/IDO2 Antibody Picoband® is an antibody targeting IDO2. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 47 kDa; calculated MW: 45424 MW.
Boster Bio Anti-INDOL1/IDO2 Antibody catalog # PA1612. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: IDO2 — Indoleamine 2,3-dioxygenase 2
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 47 kDa; Calculated: 45424 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. .
Scientific background (datasheet): IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.
Tissue details (datasheet): Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. .
Sequence similarities (datasheet): Belongs to the indoleamine 2,3-dioxygenase family.
Research relevance and current trends
- Commonly studied in contexts related to Amino Acid Metabolism,Amino Acids,Cancer,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
