| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein; EDF; INHBA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Inhibin beta A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24I91 is a rabbit monoclonal antibody that recognizes inhibin beta A chain (gene INHBA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 47.4 kDa, and the supplier reports an observed band at about 43 kDa.
Inhibin beta A (INHBA) forms the homodimer activin A and, with the alpha subunit, inhibin A. Activin A is a TGF-β family ligand that signals through type I and type II activin receptors to phosphorylate SMAD2 and SMAD3, regulating embryonic development, stem cell differentiation, reproduction, inflammation and tissue fibrosis.
| Target | Inhibin beta A chain (gene INHBA; UniProt P08476, human) |
|---|---|
| Clone | 24I91 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.4 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human U2OS cells, human PC-3 cells, human Hacat cells, human U251 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human inhibin beta A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes inhibin beta A chain as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
