| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Insulin-2;Insulin-2 B chain;Insulin-2 A chain;Ins2;Ins-2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human insulin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) is an antibody targeting INS. Common applications include IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: K36AC10; isotype: Mouse IgG1; reactivity: Human,Rabbit,Rat; observed MW: 70 kDa; calculated MW: 12339 MW.
Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.
Key elements and design rationale
- Target: INS — Insulin-2
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: K36AC10; Isotype: Mouse IgG1
- Species reactivity: Human,Rabbit,Rat
- Molecular weight guidance: Observed: 70 kDa; Calculated: 12339 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Scientific background (datasheet): Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al. (1981) and Harper and Saunders (1981) assigned the insulin gene to 11p15.5 by in situ hybridization.
Cellular localization (datasheet): Secreted.
Sequence similarities (datasheet): Belongs to the insulin family.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Chaperones,Heat Shock Proteins,Protein Trafficking,Signal Transduction,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
