| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin alpha-3; CD49 antigen-like family member C; FRP-2; Galactoprotein B3; GAPB3; VLA-3 subunit alpha; CD49c; Integrin alpha-3 heavy chain; Integrin alpha-3 light chain; ITGA3; MSK18 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Integrin alpha 3, which shares 91.2% amino acid (aa) sequence identity with both mouse and rat Integrin alpha 3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin alpha-3 (gene ITGA3) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 116.6 kDa, and the supplier reports an observed band at about 130 kDa.
Integrin alpha-3 (ITGA3, CD49c) pairs with integrin beta-1 to form a receptor for laminins, especially laminin-332 and laminin-511. It is highly expressed in epithelial cells and supports their adhesion to basement membranes, and it is important for kidney, lung and skin integrity.
| Target | Integrin alpha-3 (gene ITGA3; UniProt P26006, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 116.6 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): WB: human HeLa cells, human U87 cells, human A431 cells, human A549 cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human esophageal squamous cancer tissue, human placenta tissue, human testicular cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Synthetic peptide from the N-terminal region of human integrin alpha 3. Sequence identity with the mouse and rat orthologs: 91.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places integrin alpha-3 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Isoform 1 is widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shang-Hui Ye et al. (2026) Growth plate cartilage-targeting nanoparticles for pharmacological treatment of hypochondroplasia. Bioactive Materials. 10.1016/j.bioactmat.2026.06.018
de Queiroz Rafaela Muniz et al. (2024) Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53. Nature Communications. 10.1038/s41467-024-51488-2
Xuyang Zhang et al. (2024) Iron/ROS/Itga3 mediated accelerated depletion of hippocampal neural stem cell pool contributes to cognitive impairment after hemorrhagic stroke. Redox Biology. 10.1016/j.redox.2024.103086
Xing Yuanxin et al. (2026) Enhanced ITGA3 expression induced by helicobacter pylori infection facilitates gastric cancer progression via NF-κB and Smad4. Molecular and Cellular Biochemistry. 10.1007/s11010-026-05590-2
