| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-1;Fibronectin receptor subunit beta;Glycoprotein IIa;GPIIA;VLA-4 subunit beta;CD29;ITGB1;FNRB, MDF2, MSK12; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Integrin beta 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFI-9 is a rabbit monoclonal antibody that recognizes integrin beta-1 (gene ITGB1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 88.4 kDa, and the supplier reports an observed band at about 150 kDa.
Integrin beta-1 (ITGB1, CD29) is the most widely shared integrin beta subunit, pairing with many alpha subunits to form receptors for collagens, laminins, fibronectin and VCAM-1. Beta-1 integrins link the extracellular matrix to the actin cytoskeleton at focal adhesions and drive signaling for cell adhesion, migration, survival and tissue organization.
| Target | Integrin beta-1 (gene ITGB1; UniProt P05556, human) |
|---|---|
| Clone | AFI-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.4 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with confirmed staining (IHC): human bladder carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human integrin beta 1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin beta-1 in the cell membrane, cell projection, recycling endosome and melanosome. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells (at protein level)... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
