| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61; ITGB3; GP3A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin beta 3/ITGB3 recombinant protein (Position: N29-Q523). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes integrin beta-3 (gene ITGB3) in human and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 87.1 kDa, and the supplier reports an observed band at about 100 kDa.
Integrin beta-3 (ITGB3, CD61) pairs with alpha-IIb to form the platelet fibrinogen receptor and with alpha-V to form the vitronectin receptor αVβ3. These integrins mediate platelet aggregation, cell adhesion to RGD-containing matrix proteins, angiogenesis and bone resorption by osteoclasts. Mutations in ITGB3 cause Glanzmann thrombasthenia.
| Target | Integrin beta-3 (gene ITGB3; UniProt P05106, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 87.1 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human U-87MG cells, human HEL cells, rat spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human lung cancer tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEL cells.
Immunogen
Recombinant human Integrin beta 3/ITGB3 fragment (Asn29–Gln523), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places integrin beta-3 in the cell membrane, cell projection, cell junction and postsynaptic cell membrane. Tissue expression noted by UniProt (human): Isoform beta-3A and isoform beta-3C are widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chenggang Li et al. (2019) Effect of irisin on endometrial receptivity of rats with polycystic ovary syndrome. Gynecological Endocrinology. 10.1080/09513590.2018.1529158
