| Field | Specification |
|---|---|
| Target | |
| Alternative names | Integrin beta-4; GP150; CD antigen CD104; ITGB4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Integrin beta 4/ITGB4 recombinant protein (Position: R29-K431). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 7G10D2 is a mouse monoclonal antibody that recognizes integrin beta-4 (gene ITGB4) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 202.2 kDa, and the supplier reports an observed band at about 210 kDa.
Integrin beta-4 (ITGB4, CD104) pairs with integrin alpha-6 to form a laminin receptor that is the core of hemidesmosomes. Its long cytoplasmic tail links to keratin filaments through plectin, anchoring epithelial cells to the basement membrane, and mutations in ITGB4 cause junctional epidermolysis bullosa with pyloric atresia.
| Target | Integrin beta-4 (gene ITGB4; UniProt P16144, human) |
|---|---|
| Clone | 7G10D2 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 202.2 kDa |
| Observed MW | 210 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human A549 cells, human PC-3 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human Integrin beta 4/ITGB4 fragment (Arg29–Lys431), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places integrin beta-4 in the cell membrane and cell junction. Tissue expression noted by UniProt (human): Integrin alpha-6/beta-4 is predominantly expressed by epithelia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
