| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon regulatory factor 3;IRF-3;IRF3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human IRF3, different from the related mouse and rat sequences by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets interferon regulatory factor 3 (gene IRF3) in human samples and is validated for Western blot. The predicted molecular weight is 47.2 kDa, and the supplier reports an observed band at about 55 kDa.
Interferon regulatory factor 3 (IRF3) is a transcription factor that drives type I interferon production during viral infection. Sensing pathways such as RIG-I/MAVS, cGAS–STING and TLR3/4–TRIF activate the kinase TBK1, which phosphorylates IRF3 so it can dimerize, enter the nucleus and induce IFN-β and interferon-stimulated genes.
| Target | Interferon regulatory factor 3 (gene IRF3; UniProt Q14653, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 47.2 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human Raji cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human IRF3. It differs from the mouse and rat sequences at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon regulatory factor 3 in the cytoplasm, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Expressed constitutively in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shang Geng et al. (2024) An arms race between 5’ppp-RNA virus and its alternative recognition receptor MDA5 in RIG-I-lost teleost fish. eLife. 10.7554/eLife.94898
Shang Geng et al. (2024) An arms race between 5’ppp-RNA virus and its alternative recognition receptor MDA5 in RIG-I-lost teleost fish. eLife. 10.7554/eLife.94898.3
Yanli Su et al. (2024) Evolutionarily conserved Otub1 suppresses antiviral immune response by promoting Irf3 proteasomal degradation in miiuy croaker, Miichthys miiuy. Developmental and Comparative Immunology. 10.1016/j.dci.2024.105218
Yu Song et al. (2016) Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP+)-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway. Cell Stress & Chaperones. 10.1007/s12192-016-0695-3
