| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-3;IL-3;Hematopoietic growth factor;Mast cell growth factor;MCGF;Multipotential colony-stimulating factor;P-cell-stimulating factor;Il3;Il-3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interleukin-3 (gene Il3) in rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 18.6 kDa, and the supplier reports an observed band at about 37 kDa.
Interleukin-3 (IL-3) is a hematopoietic cytokine secreted mainly by activated T cells and mast cells. Through a receptor of IL-3Rα (CD123) and the common beta chain, it activates JAK2–STAT5 signaling to support the growth and differentiation of multiple blood cell progenitors and to activate basophils, eosinophils and monocytes.
| Target | Interleukin-3 (gene Il3; UniProt P04823, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 18.6 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Rat) |
Samples with a confirmed band (WB): PC-12 cells, NRK cells, RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant rat IL-3 fragment (Ser28–Cys166), expressed in E. coli. Sequence identity with the human and mouse orthologs: 32% and 55%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes interleukin-3 as a secreted protein. Tissue expression noted by UniProt (rat): Activated T-cells, mast cells, natural killer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
