| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Involucrin;Ivl; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human involucrin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Involucrin Ivl Antibody (Monoclonal, SY5) is an antibody targeting IVL. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: SY5; isotype: Mouse IgG1; reactivity: Human,Pig; observed MW: 68 kDa; calculated MW: 67021 MW.
Boster Bio Anti-Involucrin Ivl Antibody (Monoclonal, SY5) catalog # MA1053. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Pig.
Key elements and design rationale
- Target: IVL — Involucrin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: SY5; Isotype: Mouse IgG1
- Species reactivity: Human,Pig
- Molecular weight guidance: Observed: 68 kDa; Calculated: 67021 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Scientific background (datasheet): Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.
Cellular localization (datasheet): Cytoplasm. Constituent of the scaffolding of the cornified envelope.
Tissue details (datasheet): Keratinocytes of epidermis and other stratified squamous epithelia.
Sequence similarities (datasheet): Belongs to the insulin family.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cytoskeleton/ECM,Extracellular Matrix,Keratin,Signal Transduction,Structures,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
