| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;C7;Interferon-gamma induced protein CRG-2;Small-inducible cytokine B10;Cxcl10;Crg2, Ifi10, Inp10, Scyb10; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse IP10 recombinant protein (Position: I22-P98). Mouse IP10 shares 70.1% and 77.9% amino acid (aa) sequence identity with human and rat IP10, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Cxcl10 (C-X-C motif chemokine 10) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-IP10/CXCL10 Antibody Picoband® catalog # PB9696. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse IP10 recombinant protein (Position: I22-P98). Mouse IP10 shares 70.1% and 77.9% amino acid (aa) sequence identity with human and rat IP10, respectively. (reported region: I22-P98).
- Molecular weight context: reported MW: 19 kDa; calculated MW: 10789 MW
- Reactivity: Mouse,Rat
- Applications: ELISA, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-X-C motif chemokine 10; C-X-C motif chemokine 10. C-X-C motif chemokine 10 (CXCL10), also known as Interferon gamma-induced protein 10 kDa (IP-10) or small-inducible cytokine B10, is a protein that in humans is encoded by the CXCL10 gene. It is a small cytokine belonging to the CXC chemokine family. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. Luster et al. (1987) reported the isolation of an interferon-inducible gene that encodes a 98-amino acid protein called IP10. The chemokine IFN-gamma-inducible protein 10 (IP-10; CXCL10), a CXC chemokine, and its receptor, CXCR3, were found to be overexpressed in lymph node cells of EAMG rats. IP10 inhibits bone marrow colony formation, has antitumor activity in vivo, is a chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells. Functional note: In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development. Reported localization: Secreted. Expression/tissue context: Expressed in the spleen, thymus, lymph nodes and liver. .
Research relevance and current trends
- Angiogenesis: Researchers commonly examine how Cxcl10 (C-X-C motif chemokine 10) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how Cxcl10 (C-X-C motif chemokine 10) relates to this theme using model systems and orthogonal readouts.
- Chemokines: Researchers commonly examine how Cxcl10 (C-X-C motif chemokine 10) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Cxcl10 (C-X-C motif chemokine 10) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Cxcl10 (C-X-C motif chemokine 10) across tissue regions and cell types using matched controls.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
