| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inositol 1,4,5-trisphosphate receptor type 1;IP3 receptor isoform 1;IP3R 1;InsP3R1;Type 1 inositol 1,4,5-trisphosphate receptor;Type 1 InsP3 receptor;ITPR1;INSP3R1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IP3 receptor recombinant protein (Position: A2411-A2758). Human IP3 receptor shares 98% and 97% amino acid (aa) sequences identity with mouse and rat IP3 receptor, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 313.9 kDa, and the supplier reports an observed band at about 290 kDa.
The inositol 1,4,5-trisphosphate receptor type 1 (IP3R1, ITPR1) is a calcium channel in the endoplasmic reticulum that opens when bound by IP3 and calcium. It is especially abundant in cerebellar Purkinje cells, and mutations in ITPR1 cause several forms of spinocerebellar ataxia. It is studied in calcium signaling and neurobiology.
| Target | Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 (gene ITPR1; UniProt Q14643, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 313.9 kDa |
| Observed MW | 290 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human lung cancer tissue, human brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human IP3 receptor fragment (Ala2411–Ala2758), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98% and 97%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inositol 1,4,5-trisphosphate-gated calcium channel ITPR1 in the endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengyue You et al. (2025) Selenoprotein K-dependent MyD88 palmitoylation promotes hepatic metaflammation in high-fat diet fed mice. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2025.05.403
Miao Yu et al. (2021) LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis. Journal of Oncology. 10.1155/2021/8493431
