Anti-IP3 receptor/ITPR1 Antibody Picoband®

Overview

This antibody is intended for detection of ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-IP3 receptor/ITPR1 Antibody Picoband® catalog # PB9225. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E.coli-derived human IP3 receptor recombinant protein (Position: A2411-A2758). Human IP3 receptor shares 98% and 97% amino acid (aa) sequences identity with mouse and rat IP3 receptor, respectively. (reported region: A2411-A2758).
• Molecular weight context: reported MW: 290 kDa; calculated MW: 313929 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, IF, IHC, ICC, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Inositol 1,4,5-trisphosphate receptor type 1; Inositol 1,4,5-trisphosphate receptor type 1. Inositol 1,4,5-trisphosphate receptor type 1, also known as IP3R or IP3R1, is a protein that in humans is encoded by the ITPR1 gene. It is mapped to 3p26.1. The product of the ITPR1 gene is predominantly enriched in cerebellar Purkinje cells but is also concentrated in neurons in the hippocampal CA1 region, caudate-putamen, and cerebral cortex. The ITPR1 gene encodes the inositol 1,4,5-triphosphate (IP3) receptor, an intracellular IP3-gated calcium channel that modulates intracellular calcium signaling. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in ITPR1 cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Functional note: Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5- trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity). . Reported localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Expression/tissue context: Widely expressed.

Research relevance and current trends

• Calcium Signaling: Researchers commonly examine how ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) relates to this theme using model systems and orthogonal readouts.
• Cancer Metabolism: Researchers commonly examine how ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of ITPR1 (Inositol 1,4,5-trisphosphate receptor type 1) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the InsP3 receptor family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.