| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Occludin;OCLN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human IRAK4 Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-IRAK4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABIH-9; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-IRAK4 Monoclonal Antibody catalog # M01247. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: IRAK4 (Occludin).
- Antibody format: Monoclonal; clone ABIH-9; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
IRAK4 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. . Reported cellular localization context: Membrane; Multi-pass membrane protein. Cell junction, tight junction. Tissue expression notes (as provided): Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cardiovascular,Cytokines,Immunology,Innate Immunity,Interleukins,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,TLR Signaling,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate IRAK4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect IRAK4 expression by Western blot in cell or tissue lysates, Detect IRAK4 in FFPE tissue sections by immunohistochemistry, Localize IRAK4 by immunofluorescence/immunocytochemistry in cultured cells, Quantify IRAK4-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 59144 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 80 kDa
- Cellular localization (provided): Membrane; Multi-pass membrane protein. Cell junction, tight junction.
- Tissue details (provided): Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
