| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Interferon regulatory factor 2;IRF-2;IRF2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human IRF2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IRF2 Rabbit Monoclonal Antibody is an antibody targeting IRF2. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HAC-9; isotype: Rabbit IgG; reactivity: Human,Mouse; observed MW: 54 kDa; calculated MW: 39354 MW.
Boster Bio Anti-IRF2 Rabbit Monoclonal Antibody catalog # M02935. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: IRF2 — Interferon regulatory factor 2
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HAC-9; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 54 kDa; Calculated: 39354 MW
Biological background
Protein function (datasheet): Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Expressed throughout the epithelium of the colon. Also expressed in lamina propria. .
Research relevance and current trends
- Commonly studied in contexts related to Cytokines,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Immunology,Innate Immunity,Interferons,Pol II Transcription,Polymerase Associated Factors,Transcription,Transcription Factors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
