| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;GTPase HRas, N-terminally processed;HRAS;HRAS1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human IRF7 recombinant protein (Position: E82-A419). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-IRF7 Antibody Picoband® is an antibody for IRF7 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: IRF7 (GTPase HRas); UniProt: Q92985
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 54 kDa, calculated 21298 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-IRF7 Antibody Picoband® catalog # A00115-2.
Biological background
Biological context: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. .
Expression and localization notes: cellular localization: Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. ., tissue context: Widely expressed. ..
Common research applications
- Western blotting (WB): Compare IRF7 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of IRF7 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify IRF7-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Interferon regulatory factor 7, also known as IRF7, is a member of the interferon regulatory factor family of transcription factors. This gene is mapped to 11p15.5. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. .
- Tissue details: Widely expressed. .
- Research category: Associated Proteins,Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Differentiation,Epigenetics and Nuclear Signaling,G Protein Signaling,Intracellular,Nucleotide Messenger,Proto-Oncogenes,Ras Family,Second Messenger,Signal Transduction,Signaling Pathway,Small G Proteins,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
