| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin receptor substrate 1;IRS-1;IRS1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes insulin receptor substrate 1 (gene IRS1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 131.6 kDa, and the supplier reports an observed band at about 132 kDa.
Insulin receptor substrate 1 (IRS1) is an adaptor protein phosphorylated on tyrosine by the insulin and IGF-1 receptors. Phosphorylated IRS1 recruits PI3K and GRB2, relaying signals to the AKT and MAPK pathways that control glucose uptake, metabolism and growth. Inhibitory serine phosphorylation of IRS1 is a common readout in insulin resistance research.
| Target | Insulin receptor substrate 1 (gene IRS1; UniProt P35568, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 131.6 kDa |
| Observed MW | 132 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): A549 cells, MM453 cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, rat skeletal muscle tissue, human intestinal cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human IRS1 fragment (Ser1041–Gln1242), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 78% and 80%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places insulin receptor substrate 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xi Wang et al. (2025) Tea polyphenols ameliorates insulin resistance and inflammation by modulating macrophage polarization via JAK2/STAT3 pathway in aged T2DM combined with NAFLD rats. Journal of Functional Foods. 10.1016/j.jff.2025.106727
Shuangzhi Chen et al. (2025) Targeting to Intensify M2 Macrophage Polarization Ameliorate Podocyte Lipid Accumulation and Damage by Tea Polyphenols via Activating SIRT1 in the Aged Model Rats With DKD. Food Science & Nutrition. 10.1002/fsn3.70736
Muyao Wang et al. (2023) The impact of irradiation on ginsenoside variations in red ginseng and its hypoglycemic effects in type 1 diabetic mice. Journal of Agriculture and Food Research. 10.1016/j.jafr.2023.100753
Lin Mi et al. (2014) MicroRNA-139-5p Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch and IRS1/PI3K/Akt Insulin Signaling Pathways. Journal of Cellular Biochemistry. 10.1002/jcb.25065
Ying Liu et al. (2015) Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model. International Journal of Clinical and Experimental Medicine. PMID: 26550236
Gui-You Liang et al. (2017) Role of insulin receptors in myocardial ischaemia-reperfusion injury during cardiopulmonary bypass. ACTA Cardiologica. 10.1080/AC.66.3.2114132
