| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; HECT-type E3 ubiquitin transferase Itchy homolog; NFE2-associated polypeptide 1; NAPP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ITCH |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23I97 is a rabbit monoclonal antibody that recognizes E3 ubiquitin-protein ligase Itchy homolog in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 102.8 kDa, and the supplier reports an observed band at about 103 kDa.
ITCH is a HECT-type E3 ubiquitin ligase of the NEDD4 family. It tags many substrates for degradation or altered signaling, including JUNB, c-FLIP and components of TNF and TLR pathways, and it is important for limiting inflammation and preventing autoimmunity, as ITCH-deficient mice develop severe inflammatory disease.
| Target | E3 ubiquitin-protein ligase Itchy homolog (gene ITCH; UniProt Q96J02, human) |
|---|---|
| Clone | 23I97 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 102.8 kDa |
| Observed MW | 103 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ITCH.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 ubiquitin-protein ligase Itchy homolog in the cell membrane, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
