| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Inositol 1,4,5-trisphosphate receptor type 3;IP3 receptor isoform 3;IP3R 3;InsP3R3;Type 3 inositol 1,4,5-trisphosphate receptor;Type 3 InsP3 receptor;ITPR3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ITPR3 Antibody Picoband® catalog # PB9428. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 270-300 kDa; calculated MW: 304106 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Inositol 1,4,5-trisphosphate receptor type 3; Inositol 1,4,5-trisphosphate receptor type 3. ITPR3 encodes a receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. The receptor contains a calcium channel at the C-terminus and the ligand-binding site at the N-terminus. Knockout studies in mice suggest that type 2 and type 3 inositol 1,4,5-trisphosphate receptors play a key role in exocrine secretion underlying energy metabolism and growth. ITP3 channels serve an important role in the taste transduction pathway of sweet, bitter and umami tastes the gustatory system. ITP3 channels allow the flow of Calcium out of the endoplasmic reticulum in response to IP3. Calcium cations result in the activation of TRPM5 which leads to a depolarisation generating potential and an action potential. Functional note: Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Reported localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Expression/tissue context: Expressed in intestinal crypt and villus epithelial cells.
Research relevance and current trends
- Calcium Channels: Researchers commonly examine how ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ITPR3 (Inositol 1,4,5-trisphosphate receptor type 3) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the InsP3 receptor family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
