| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inositol 1,4,5-trisphosphate receptor type 3;IP3 receptor isoform 3;IP3R 3;InsP3R3;Type 3 inositol 1,4,5-trisphosphate receptor;Type 3 InsP3 receptor;ITPR3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ITPR3, identical to the related mouse and rat sequences. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody targets inositol 1,4,5-trisphosphate-gated calcium channel ITPR3 in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 304.1 kDa, and the supplier reports an observed band at about 270–300 kDa.
The inositol 1,4,5-trisphosphate receptor type 3 (IP3R3, ITPR3) is an ER calcium release channel activated by IP3. It is prominent in epithelial cells such as secretory and taste cells, and it also transfers calcium to mitochondria at ER–mitochondria contact sites, influencing metabolism and cell death.
| Target | Inositol 1,4,5-trisphosphate-gated calcium channel ITPR3 (gene ITPR3; UniProt Q14573, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 304.1 kDa |
| Observed MW | 270–300 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human ITPR3. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places inositol 1,4,5-trisphosphate-gated calcium channel ITPR3 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in intestinal crypt and villus epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sen-Yuan Hong et al. (2023) Inhibition of AT1R/IP3/IP3R-mediated Ca2+ release protects against calcium oxalate crystals-induced renal oxidative stress. Chemico-Biological Interactions. 10.1016/j.cbi.2023.110636
