| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein jagged-2; Jagged2; hJ2; JAG2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human JAG2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25J98 is a rabbit monoclonal antibody that recognizes protein jagged-2 (gene JAG2) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 133.4 kDa, and the supplier reports an observed band at about 145 kDa.
Jagged-2 (JAG2) is a cell surface ligand for Notch receptors. It signals through Notch to shape cell fates during development, including limb, craniofacial, thymus and inner ear development, and it is studied in Notch signaling and lymphocyte development.
| Target | Protein jagged-2 (gene JAG2; UniProt Q9Y219, human) |
|---|---|
| Clone | 25J98 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 133.4 kDa |
| Observed MW | 145 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HUVEC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human JAG2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places protein jagged-2 in the membrane. Tissue expression noted by UniProt (human): Expressed in heart, placenta and skeletal muscle and to a lesser extent in pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
