| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
The antibody reacts specifically with the activated diphosphorylated form of JNK. The epitope recognized by the antibody contains the regulatory site of JNK (Thr183 and Tyr185). Weak cross-reaction is observed with the monophosphorylated threonine peptide, but not with the non-phosphorylated or monophosphorylated tyrosine peptides of JNK. It does not recognize any form of p38-MAPK or ERK-MAPK.
Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse clone JNK-PT48, ~2 mg/mL, purified immunoglobulin, buffered aqueous solution
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | See datasheet |
| ELISA | See datasheet |
| ICC/IF | See datasheet |
| Other | See datasheet |
Product Specifications
| Clone | JNK-PT48 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 |
| Species Reactivity | Human, Mouse, Rat |
| Purification | purified immunoglobulin |
| Concentration | 1ug/ul |
| Formulation | Liquid |
| Storage | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Immunogen / Antigen
synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB, ELISA, ICC/IF, Other. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
