| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human K Cadherin/CDH6 recombinant protein (Position: N153-H424). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-K Cadherin/CDH6 Antibody Picoband® is an antibody for CDH6 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CDH6 (Rho-related GTP-binding protein Rho6); UniProt: P55285
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 88 kDa, calculated 26056 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-K Cadherin/CDH6 Antibody Picoband® catalog # A06353-1.
Biological background
Biological context: Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments. .
Expression and localization notes: cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton ., tissue context: Mostly expressed in brain and liver..
Common research applications
- Western blotting (WB): Compare CDH6 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CDH6 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CDH6-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton .
- Tissue details: Mostly expressed in brain and liver.
- Research category: Actin Assembly,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,G Protein Signaling,Microfilaments,Ras Family,Signal Transduction,Signaling Pathway,Small G Proteins
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
