| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kallistatin; Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4; SERPINA4; KST; PI4 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Kallistatin/PI-4/SERPINA4 recombinant protein (Position: H147-A373). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® is an antibody for SERPINA4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SERPINA4 (serpin family A member 4); UniProt: P29622
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 49 kDa, calculated 82457 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® catalog # A08426.
Biological background
Biological context: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
Expression and localization notes: cellular localization: Secreted., tissue context: Expressed by the liver and secreted in plasma..
Common research applications
- Western blotting (WB): Compare SERPINA4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SERPINA4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SERPINA4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Kallistatin is a protein that in humans is encoded by the SERPINA4 gene. It is mapped to 14q32.13. Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors. Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Expressed by the liver and secreted in plasma.
- Research category: Cancer,Cell Biology,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Metalloprotease,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
