| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kallistatin;Kallikrein inhibitor;Peptidase inhibitor 4;PI-4;Serpin A4;SERPINA4;KST, PI4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Kallistatin/SERPINA4 Antibody Picoband® is an antibody targeting SERPINA4. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 91 kDa; calculated MW: 48542 MW.
Boster Bio Anti-Kallistatin/SERPINA4 Antibody catalog # PA1638. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: SERPINA4 — Kallistatin
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 91 kDa; Calculated: 48542 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .
Scientific background (datasheet): Serpina 4, also called Kallistatin, PI4 or TISSUE KALLIKREIN INHIBITOR, is a protein that in humans is encoded by the SERPINA4 gene. The serpina4 is a member of the serpin superfamily and represents a major inhibitor of human tissue kallikrein in the circulation. The SERPINA4 gene is localized to 14q32.13. The translated amino acid sequence of serpina4 matches the protein sequence and shares 44 to 46% sequence identity with other serpin family members. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. And serpina4 also plays a role in neointima hyperplasia.
Cellular localization (datasheet): Secreted.
Tissue details (datasheet): Expressed by the liver and secreted in plasma.
Sequence similarities (datasheet): Belongs to the serpin family.
Research relevance and current trends
- Commonly studied in contexts related to Angiogenesis,Cancer,Cardiovascular,Cell Biology,Invasion/Microenvironment,Protease Inhibitors,Proteolysis/Ubiquitin,Serum Proteins.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
