Anti-KAT13A / SRC1 Monoclonal Antibody

Overview

This product is an anti-NCOA1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEHD-14; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-KAT13A / SRC1 Monoclonal Antibody catalog # M00856. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Key elements and design rationale

• Target: NCOA1 (Angiopoietin-1).
• Antibody format: Monoclonal; clone AEHD-14; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

NCOA1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. . Reported cellular localization context: Secreted. Tissue expression notes (as provided): KMT4 / Dot1L Antibody detects endogenous levels of total KMT4 / Dot1L

Research relevance and current trends

• Research context keywords from the source record include: Acetylation,Cancer,Cancer Metabolism,2339,Chromatin Modifying Enzymes,Co-Activators/Co-Repressors,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Nuclear Hormone Receptors,Nuclear Receptors,Nuclear Signaling Pathways,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Signal Transduction,Signaling Pathway,Transcription.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate NCOA1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NCOA1 expression by Western blot in cell or tissue lysates, Detect NCOA1 in FFPE tissue sections by immunohistochemistry, Enrich NCOA1 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 157 kDa; calculated MW: 57513 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 157 kDa
• Cellular localization (provided): Secreted.
• Tissue details (provided): KMT4 / Dot1L Antibody detects endogenous levels of total KMT4 / Dot1L

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.