| Field | Specification |
|---|---|
| Target | |
| Alternative names | Elongator complex protein 3; hELP3; tRNA uridine(34) acetyltransferase; ELP3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KAT9 / Elp3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADAF-5 is a rabbit monoclonal antibody that recognizes elongator complex protein 3 (gene ELP3) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 62.3 kDa, and the supplier reports an observed band at about 62 kDa.
ELP3 (also called KAT9) is the catalytic subunit of Elongator, the complex that installs carboxymethyl-based modifications on the wobble uridine of tRNAs to support accurate and efficient translation. The protein is conserved from yeast to human, and in mammals it is also studied for its roles in cortical neuron migration and neurodevelopment.
| Target | Elongator complex protein 3 (gene ELP3; UniProt Q9H9T3, human) |
|---|---|
| Clone | ADAF-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 62.3 kDa |
| Observed MW | 62 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human KAT9 / Elp3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places elongator complex protein 3 in the cytoplasm, nucleus and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in the cerebellum and spinal motor neurons.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
