| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hemoglobin subunit gamma-1;Gamma-1-globin;Hb F Agamma;Hemoglobin gamma-1 chain;Hemoglobin gamma-A chain;HBG1;PRO2979; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human KAT9 / Elp3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ELP3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADAF-5; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-KAT9 / Elp3 Rabbit Monoclonal Antibody catalog # M02833. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ELP3 (Hemoglobin subunit gamma-1).
- Antibody format: Monoclonal; clone ADAF-5; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ELP3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Gamma chains make up the fetal hemoglobin F, in combination with alpha chains. Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Red blood cells.
Research relevance and current trends
- Research context keywords from the source record include: Acetylation,Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling,Pol II Transcription,Polymerase Associated Factors,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate ELP3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ELP3 expression by Western blot in cell or tissue lysates, Localize ELP3 by immunofluorescence/immunocytochemistry in cultured cells, Enrich ELP3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 12 kDa; calculated MW: 16140 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 12 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Red blood cells.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
