| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium voltage-gated channel subfamily A member 5;HPCN1;Voltage-gated potassium channel HK2;Voltage-gated potassium channel subunit Kv1.5;KCNA5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human KCNA5, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects potassium voltage-gated channel subfamily A member 5 (gene KCNA5) in human samples and is validated for Western blot. The predicted molecular weight is 67.2 kDa, and the supplier reports an observed band at about 67 kDa.
Kv1.5 (KCNA5) is a Shaker-type voltage-gated potassium channel that activates quickly and inactivates slowly. In the human heart it carries the ultra-rapid delayed rectifier current (IKur) in the atria, which contributes to atrial repolarization, and it is also expressed in vascular smooth muscle. Kv1.5 is studied in atrial fibrillation and pulmonary vascular research.
| Target | Potassium voltage-gated channel subfamily A member 5 (gene KCNA5; UniProt P22460, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 67.2 kDa |
| Observed MW | 67 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): 293T cells, A549 cells, PANC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human KCNA5. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places potassium voltage-gated channel subfamily A member 5 in the cell membrane. Tissue expression noted by UniProt (human): Pancreatic islets and insulinoma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ya Lu et al. (2022) MicroRNA153 induces apoptosis by targeting NFATc3 to improve vascular remodeling in pulmonary hypertension. Clinical and Experimental Hypertension. 10.1080/10641963.2022.2140810
