| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein S100-A10; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11; S100A10; ANX2LG; CAL1L; CLP11 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human KCNJ1, which shares 80% amino acid (aa) sequence identity with both mouse and rat KCNJ1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-KCNJ1 Antibody Picoband® is an antibody for KCNJ1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Monkey,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: KCNJ1 (S100 calcium binding protein A10); UniProt: P48048
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 45 kDa, calculated 12252 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-KCNJ1 Antibody Picoband® catalog # A02788-2.
Biological background
Biological context: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase.
Expression and localization notes: cellular localization: alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; cell body; cell-cell junction; dendritic spine; integral component of membrane.
Common research applications
- Western blotting (WB): Compare KCNJ1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of KCNJ1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify KCNJ1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; cell body; cell-cell junction; dendritic spine; integral component of membrane
- Research category: Signal Transduction,Signaling Pathway,Cytoskeleton/ECM,Calcium Binding Proteins,Calcium Signaling,Cell Adhesion
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
