| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lysine-specific demethylase 5A; Histone demethylase JARID1A; Jumonji/ARID domain-containing protein 1A; Retinoblastoma-binding protein 2; RBBP-2; [histone H3]-trimethyl-L-lysine(4) demethylase 5A; KDM5A; JARID1A; RBBP2; RBP2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human KDM5A/Jarid1A/RBBP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEHC-11 is a rabbit monoclonal antibody that recognizes lysine-specific demethylase 5A (gene KDM5A) in human and mouse samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 192.1 kDa, and the supplier reports an observed band at about 192 kDa.
KDM5A (JARID1A, RBP2) is a JmjC domain histone demethylase that removes tri- and dimethyl marks from histone H3 at Lys4, a mark found at active promoters. It binds DNA and partners such as the retinoblastoma protein to regulate gene expression during differentiation. KDM5A is studied in epigenetic regulation and in cancer drug tolerance.
| Target | Lysine-specific demethylase 5A (gene KDM5A; UniProt P29375, human) |
|---|---|
| Clone | AEHC-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 192.1 kDa |
| Observed MW | 192 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:80 |
| Flow cytometry | 1:200 |
Samples with a confirmed band (WB): HEK293 cells, mouse spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human KDM5A/Jarid1A/RBBP2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places lysine-specific demethylase 5A in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
