| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kelch-like ECH-associated protein 1;Cytosolic inhibitor of Nrf2;INrf2;Kelch-like protein 19;KEAP1;INRF2, KIAA0132, KLHL19; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes kelch-like ECH-associated protein 1 (gene KEAP1) in human samples and is validated for Western blot. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 66 kDa.
KEAP1 is a substrate adapter for a CUL3-based E3 ubiquitin ligase that keeps the transcription factor NRF2 (NFE2L2) at low levels under normal conditions. Reactive cysteines in KEAP1 act as sensors of oxidants and electrophiles; when they are modified, NRF2 escapes degradation and turns on antioxidant and detoxifying genes. The KEAP1–NRF2 pathway is central to oxidative stress research, and KEAP1 is frequently mutated in lung cancer.
| Target | Kelch-like ECH-associated protein 1 (gene KEAP1; UniProt Q14145, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 69.7 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human HepG2 cells, human A549 cells, human PC-3 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Keap1 fragment (Glu25–Glu205), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.7% and 97.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places kelch-like ECH-associated protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Broadly expressed, with highest levels in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiemei Chen et al. (2022) Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora. Pharmacological Research. 10.1016/j.phrs.2022.106191
Yun-Tao Zhao et al. (2022) Tilapia skin peptides restore cyclophosphamide-induced premature ovarian failure via inhibiting oxidative stress and apoptosis in mice. Food & Function. 10.1039/D1FO04239D
Xiaorui Lv et al. (2023) Human Neural Stem Cell Secretome Inhibits Neuron Heme Uptake and Ferroptosis in Intracerebral Hemorrhage Through Nrf-2 Signaling Pathway. Stem Cells and Development. 10.1089/scd.2023.0010
Qing Zeng et al. (2021) Resveratrol attenuates methamphetamine-induced memory impairment via inhibition of oxidative stress and apoptosis in mice. Journal of Food Biochemistry. 10.1111/jfbc.13622
