| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kinesin-like protein KIF15; Kinesin-like protein 2; hKLP2; Kinesin-like protein 7; Serologically defined breast cancer antigen NY-BR-62; KIF15; KLP2; KNSL7 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-KIF15 Antibody Picoband® is an antibody for KIF15 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: KIF15 (kinesin family member 15); UniProt: Q9NS87
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 150-160 kDa, calculated 80420 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-KIF15 Antibody Picoband® catalog # A05983-1.
Biological background
Biological context: Plus-end ed kinesin-like motor enzyme involved in mitotic spindle assembly.
Expression and localization notes: cellular localization: Spindle. Cytoplasm., tissue context: Expressed in testis, colon, thymus and in breast cancer..
Common research applications
- Western blotting (WB): Compare KIF15 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of KIF15 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify KIF15-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Kinesin family member 15 is a protein that in humans is encoded by the KIF15 gene. It is mapped to 3p21.31. This gene encodes a motor protein that is part of the kinesin superfamily. KIF15 maintains half spindle separation by opposing forces generated by other motor proteins. KIF15 co-localizes with microtubules and actin filaments in both dividing cells and in postmitotic neurons. KIF15 (also known as Kinesin-12 and HKLP2) is a motor protein expressed in all cells during mitosis and in postmitotic neurons undergoing axon growth. KIF15 maintains bipolar microtubule spindle apparatus in dividing cells and shares redundant functions with KIF11. KIF15 is thought to promote spindle assembly by cross-linking and sliding along microtubules creating a separation between centrosomes. HeLa cells depleted of KIF11, with reduced microtubule dynamics, are able to form bipolar spindles from acentrosomal asters in a KIF15 dependent manner. Hence, inhibition of KIF15 function will be a vital therapeutic approach in cancer chemotherapy.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Spindle. Cytoplasm.
- Tissue details: Expressed in testis, colon, thymus and in breast cancer.
- Research category: Cell Biology,Cell Cycle,Cell Division,Cytoskeleton,Cytoskeleton/ECM,DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling,Motor Proteins,Organelles,Signal Transduction,Subcellular Markers,Tags & Cell Markers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
