Anti-Kir6.1/KCNJ8 Antibody Picoband®

Overview

Anti-Kir6.1/KCNJ8 Antibody Picoband® is an antibody for KCNJ8 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: KCNJ8 (Prolactin regulatory element-binding protein); UniProt: Q15842
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 48 kDa, calculated 45468 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Kir6.1/KCNJ8 Antibody Picoband® catalog # A04950-1.

Biological background

Biological context: Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER. .

Expression and localization notes: cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. ., tissue context: Ubiquitous. ..

Common research applications

• Western blotting (WB): Compare KCNJ8 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of KCNJ8 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify KCNJ8-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Potassium inwardly-rectifying channel, subfamily J, member 8, also known as KCNJ8, is a human gene encoding the Kir6.1 protein. Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS).
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Endoplasmic reticulum membrane ; Single-pass membrane protein . Nucleus . Concentrates at endoplasmic reticulum exit sites. .
• Tissue details: Ubiquitous. .
• Research category: Epigenetics and Nuclear Signaling,Transcription

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.